Time-lapse two-photon imaging of the calcium indicator, GCaMP6f, in an acute slice of a 11-day-old mouse olfactory bulb (depth, 150 μm). GCaMP6f was expressed in mitral cells. The medium was exchanged ...
Novel, ring-inserts' are prepared (1) and loaded with growth factors of interest using microcontact printing (2). Brain slices taken from postnatal day 8-10 mice are added (3) and nerve fibers grow ...